Tsis ntev los no, US Environmental Protection Agency (EPA) tau pom zoo tias RNAi cov khoom siv tshuaj tua kab ledprona uas muaj cov khoom xyaw tshiab tau sau npe rau peb xyoos. Lub sijhawm no yog raws li EPA lub sijhawm sau npe rau lwm yam khoom siv tshuaj tua kab tshiab.
Ledprona yog cov tshuaj tua kab tshiab uas tso siab rau RNA cuam tshuam (RNAi), ib qho cuab yeej siv los ntawm cov nroj tsuag thiab kab los tua kab mob. Lub tswv yim tshiab biopesticide yog tshuaj tsuag ob-stranded ribonucleic acid (dsRNA) cov khoom lag luam uas tsom rau Colorado qos beetle (CPB). CPB yog cov kab tsuag tseem ceeb ntawm cov qos yaj ywm hauv Tebchaws Meskas. Nws feem ntau pub rau qos yaj ywm nplooj nplooj. Nws tuaj yeem txhim kho kev tiv thaiv tshuaj tua kab sai sai, ua rau nws nyuaj rau tswj thiab ua rau muaj kev phom sij heev.
Ledprona siv RNA cuam tshuam los tshuaj tsuag dsRNA cov khoom los tua cov kab tsuag los ntawm "silencing" CPB noob yuav tsum tau tsim cov protein PSMB5. Lub luag haujlwm ntawm PSMB5 protein yog qhov tseem ceeb rau kev tswj hwm lub neej ntawm CPB yam tsis ua rau muaj qhov tshwm sim ntawm cov kab mob hloov pauv. Yog tias pom zoo los ntawm EPA, cov tshuaj tua kab RNAi no yuav yog lub ntiaj teb thawj cov tshuaj tua kab dsRNA tso cai rau kev lag luam siv thiab txau rau cov nroj tsuag.
Tam sim no, EPA tau ua qhov kev ntsuam xyuas dav dav ntawm Ledprona los xyuas kom meej tias cov khoom siv biotechnology tshiab no tsis ua rau muaj kev cuam tshuam tsis zoo rau ib puag ncig thiab tias nws cov khoom seem tsis ua rau muaj kev phom sij rau tib neeg cov zaub mov. Nyob rau lub Tsib Hlis ntawm lub xyoo no, peb tau sib koom ua ib qho kev ntsuam xyuas nruj ntawm cov khoom siv biotechnology tshiab no nrog RNAi cov kws paub txog tshuaj tua kab uas tsim los ntawm OECD Pesticide Working Group thiab cov koom tes thoob ntiaj teb thiab cov kws tshaj lij hauv thaj chaw. Raws li Tsoom Fwv Teb Chaws Tshuaj tua kab, Fungicide thiab Rodenticide Act (FIFRA), cov khoom tau raug pom zoo rau Kev Tso Cai Siv Tshuaj (EUP) hauv 10 lub xeev hauv Tebchaws Meskas. Txog rau tam sim no, tsis pom qhov cuam tshuam txog kev nyab xeeb.
dsRNA tam sim no tsim los ntawm Zhi Sheng Yougu muaj purity siab, thiab tom qab kev kho kom ruaj khov, nws cov dej num tuaj yeem tswj tau ntau dua 15 hnub thiab nws ib nrab-lub neej yog 20-30 hnub. Nws tseem muaj kev ruaj ntseg zoo nyob rau hauv cov xwm txheej ntawm lub teeb muaj zog irradiation, ultraviolet irradiation, thiab kho acid thiab alkali. Kev ntsuam xyuas teb tau ua rau cov kab tsuag qoob loo uas muaj zog tiv thaiv cov tshuaj tua kab. Piv txwv li, tus nqi tuag rau aphids yog sib npaug ntawm cov tshuaj tua kab ib txwm muaj, txog li 90%; rau cov kab kab daj daj, qhov kev tuag nce mus txog 92% hauv 24 teev.
Nyob rau hauv lub neej yav tom ntej, Plant Biotech yuav txuas ntxiv mus siv nws lub luag haujlwm ntawm "tsim tshuaj tua kab ntsuab thiab ua haujlwm rau kev ua liaj ua teb ntsuab", muab kev ua qauv qhia rau kev tsim thiab siv cov tshuaj tua kab ntsuab tshiab hauv kuv lub tebchaws, thiab muab cov qauv rau kuv lub teb chaws cov khoom tshiab RNA biotechnology. hauv kev ruaj ntseg zaub mov, cog kev nyab xeeb thiab "ob npaug-siv". thiab ua kom muaj txiaj ntsig zoo rau kev ua tiav ntawm cov hom phiaj carbon.
